Coding
Bioplastic

Part:BBa_K156012:Design

Designed by: George McArthur IV   Group: iGEM08_Virginia   (2008-10-28)

phaA (acetyl-CoA acetyltransferase)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 886
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 852
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 225
    Illegal NgoMIV site found at 237
    Illegal AgeI site found at 600
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was codon-optimized for expression in E. coli. The following restriction enzyme sites were also removed: EcoRI, NotI, XbaI, SpeI, PstI, NheI.


Source

This coding region is native to Ralstonia eutropha H16. The original sequence was obtained from GenBank (VERSION NC_008313.1, GI:113866031).

References

Pohlmann, A. et al. (2006). Genome sequence of the bioplastic-producing "Knallgas" bacterium Ralstonia eutropha H16. Nat Biotechnol. 24(10):1257-62.